{"id":15793,"date":"2019-02-04T12:51:09","date_gmt":"2019-02-04T12:51:09","guid":{"rendered":"http:\/\/organistry.com.mialias.net\/our-services\/hplc-preparativo\/"},"modified":"2019-02-25T14:03:53","modified_gmt":"2019-02-25T14:03:53","slug":"hplc-preparative","status":"publish","type":"page","link":"https:\/\/www.organistry.es\/en\/our-services\/hplc-preparative\/","title":{"rendered":"Purification of impurities by preparative HPLC"},"content":{"rendered":"<section class=\"wpb-content-wrapper\"><p>[vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; css=&#8221;.vc_custom_1549539259242{padding-top: 50px !important;padding-bottom: 30px !important;}&#8221; el_class=&#8221;tld-lista-decorada&#8221;][vc_column][vc_row_inner el_class=&#8221;tld-two-blocks tld-two-blocks-reverse&#8221;][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]We have <strong>the best technology to carry out purifications and isolations in preparative HPLC<\/strong>: Waters equipment with automatic injector and fraction collector.<\/p>\n<p>We have the same column chemistry as in the analytical HPLC. This leads to a transfer of analytical conditions to preparative in an easy and fast way. We also develop new methods that avoid the use of non-volatile salts, especially in the case of very polar products of interest.<\/p>\n<p>We use our equipment for:<\/p>\n<ul>\n<li><strong>Purification on C18 or HILIC columns (very polar products)<\/strong>: to reach a purity specification that is not easily obtained by other purification routes (such as crystallization or SiO<sub>2<\/sub> chromatography, also available in Organistry).<\/li>\n<li><strong>Isolation of products in complex mixtures<\/strong>: to obtain a product whose synthesis is not viable or is not efficient (many synthetic stages).<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;15489&#8243; img_size=&#8221;large&#8221; alignment=&#8221;right&#8221; css_animation=&#8221;right-to-left&#8221;][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner el_class=&#8221;tld-two-blocks tld-two-blocks-reverse&#8221;][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]The isolation also allows the availability of a standard of an unknown impurity whose structure has to be established unequivocally by NMR (when by HR-MS it is established that there are several likely isomers).<\/p>\n<p>In the case of degradation impurities, before starting the preparative work, <strong>forced degradation experiments are designed to increase the impurity content<\/strong> without the mixture becoming too complex. The percentage of impurity generated will impact in the yield of the separation and in the delivery time.<\/p>\n<p>We work in the <strong>scale of 10 mg &#8211; 5 g of product<\/strong>, depending on the starting purity and the requirements of the client.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;15491&#8243; img_size=&#8221;large&#8221; alignment=&#8221;right&#8221; css_animation=&#8221;right-to-left&#8221;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; bg_type=&#8221;image&#8221; parallax_style=&#8221;vcpb-vz-jquery&#8221; bg_image_new=&#8221;id^15908|url^https:\/\/www.organistry.es\/wp-content\/uploads\/2019\/02\/CTA-hplc-2.jpg|caption^null|alt^null|title^CTA-hplc|description^null&#8221; bg_image_repeat=&#8221;no-repeat&#8221; el_class=&#8221;tld-cta tld-cta-green tld-cta-two-col&#8221;][vc_column width=&#8221;3\/4&#8243;][vc_custom_heading text=&#8221;We isolate your impurity in a complex mixture,&#8221; font_container=&#8221;tag:h3|text_align:center&#8221; use_theme_fonts=&#8221;yes&#8221;][vc_custom_heading text=&#8221;do you want to know how we get it?&#8221; font_container=&#8221;tag:h2|text_align:center&#8221; use_theme_fonts=&#8221;yes&#8221;][\/vc_column][vc_column width=&#8221;1\/4&#8243;][vc_btn title=&#8221;Know more&#8221; link=&#8221;url:http%3A%2F%2Forganistry.com.mialias.net%2Fen%2Fcontact%2F|||&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"<p>[vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; css=&#8221;.vc_custom_1549539259242{padding-top: 50px !important;padding-bottom: 30px !important;}&#8221; el_class=&#8221;tld-lista-decorada&#8221;][vc_column][vc_row_inner el_class=&#8221;tld-two-blocks tld-two-blocks-reverse&#8221;][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]We have the best technology to carry out purifications and isolations in preparative&#8230;<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":15760,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-15793","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/pages\/15793","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/comments?post=15793"}],"version-history":[{"count":7,"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/pages\/15793\/revisions"}],"predecessor-version":[{"id":15911,"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/pages\/15793\/revisions\/15911"}],"up":[{"embeddable":true,"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/pages\/15760"}],"wp:attachment":[{"href":"https:\/\/www.organistry.es\/en\/wp-json\/wp\/v2\/media?parent=15793"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}